Monday, September 22, 2008

Human Skin Fibroblast 1184- husna




Picture (R)- HSF 1184 after 1 day revival
Picture (L)- HSF 1184 at 100% confluent
I use human skin fibroblast 1184 in order to achieve my research objective to determine the
cytotoxicity of papain-based wound cleanser to the healthy cells around the wounded area. HSF 1184 will also be used in order to investigate the cleanser’s effectiveness in promoting wound regeneration based on in vitro wound assay.

Human skin fibroblast 1184 was purchased from European Collection of Cell Culture (ECACC). It was derived from human skin by none other than Dr C. Arlett from the MRC Cell Mutation Unit, University of Sussex.

I’ve handled HSF 1184 since 21st August 2008. Picture attached here shows the cells after 1 day revival. For the first revival, it took about 7 to 8 days for the cells to become 70-80% confluent. It was tricky at first since I wasn’t sure about the cells’ pattern. It took me a while to get used to the cells thus the experience was indeed priceless.

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